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10L Media Kit: Bristol Medium10L Media Kit: Bristol Medium H. C. Bold's modification of Bristol's recipe (Bold 1949). General purpose freshwater medium that lacks vitamins and is the essential base component for other media such as Bold 3N and Modified Bold 3N Media (see table below). Media kit includes all the necessary chemical components to prepare 10 Liters of Bristol Medium. All UTEX Media Kit solutions are shipped sterile and should be handled aseptically to avoid
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10L Media Kit: Bristol Medium

H.C. Bold's modification of Bristol's recipe (Bold 1949). General purpose freshwater medium that lacks vitamins and is the essential base component for other media such as Bold 3N and Modified Bold 3N Media (see table below).

Media kit includes all the necessary chemical components to prepare 10 Liters of Bristol Medium. All UTEX Media Kit solutions are shipped sterile and should be handled aseptically to avoid contamination.


What is a media kit? How is it different than stock solutions?

UTEX Media Kits come with pre-measured, ready-to-use components. Unlike stock solutions, there is no need to prepare and/or dilute concentrated reagents for each individual chemical at precise ratios. Simply follow the included instructions to create the large volume of media—precision without the hassle of extra preparation.

Do the media kits include stock solutions?

No. UTEX Media Kits do not include concentrated stock solutions for each individual chemical component of a medium. Compatible chemicals have been pre-measured and combined to not only make preparation easier, but also reduce packaging and shipping costs. 

What if I want individual stock solutions?

Each culture medium page includes a recipe that, when applicable, provides instructions for preparing stock solutions and how much to add. Because some components aren’t suitable for stock solutions, related instructions are provided only when applicable.


Looking for Bristol Medium in 1-Liter and test tube volumes?

View/purchase prepared and sterile 1X Bristol Medium


Available Bristol Media Kit Upgrades

For similar chemical components but with 3X the nitrogen and the addition of vitamins, view the available Bold 3N and Modified Bold 3N upgrades for this kit. You can purchase the Bristol Media Kit with an upgrade at a discount using the 'Options' dropdown at the top of the page. Media Upgrade Kits are also available to purchase separately on their respective pages.

Medium At A Glance

  • Water Type Freshwater
    pH of Medium 6<ph<8
    Contains NO3



Directions

For 10L Bristol Medium Total Volume

  1. Obtain and wear the appropriate PPE.
  2. To approximately 8.4 Liters (8,400 mL) of dH2O, carefully add the entire bottle of Bristol solution while stirring.
    • Bring total volume to 10 Liters (10,000 mL) with dH2O.
    • Cover and autoclave or filter-sterilize if non-sterile water was used.
    • Store at refrigerator temperatures.
    Chemical
    Final Concentration
    NaNO3 2.94 mM
    CaCl2•2H2O 0.17 mM
    MgSO4•7H2O 0.3 mM
    K2HPO4 0.43 mM
    KH2PO4 1.29 mM
    NaCl 0.43 mM

    10L Media Kit: Bristol Medium

    Item no : 9456147976
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    US$ 155.00
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